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descargar curso de piano orbis fabbriThe CORE project is an ongoing research project investigating the subcellular localization of three different types of RNA polymerase (RNAP) in oocytes of Xenopus laevis. We have started with purification of the RNAP from the different stages of oogenesis. The RNA products from each RNAP will be characterized by RNA-sequence analysis and the localization of the RNAPs will be correlated with the transcription of the X. laevis-specific components of the genome. The purified RNAPs from the different stages of oogenesis will also be used for production of antibodies. The anti-RNAP will serve as the starting point to produce highly specific anti-RNAP antibodies that will be used for immunostaining during oogenesis to study the localization of RNA polymerases in Xenopus oocytes. The RNAPs purified in the CORE project will also be used to produce antiserum to localize the different nuclear RNAPs in interphase nuclei of early embryos. Both the RNAP and the immunolocalization project will involve studies of the functional elements of the genome. The RNAP will be purified without any interference with the biochemical and immunological functions of the enzyme. Genomic segments of the X. laevis-specific DNA that contain the regulatory regions of the genome such as the oocyte- specific regulon will be cloned in bacteriophage vectors and used for expression of the gene encoding RNAP in the E. coli. The site and level of expression of RNAP in oocytes and in early embryos will be studied by following the mRNA in situ by in situ hybridization.Q:
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FK Miljevci () is a football club based in the village of Miljevci, near Šabac, Serbia. They currently compete in the Serbian Third League, the third tier of the national league system.
History
The club was founded in 1992 and first competed in the Serbian League of the Third Level in the 2002–03 season. They finished bottom of the table in both the 2003–04 and 2004–05 seasons, and were subsequently relegated to the regional Serbian League of Vojvodina, the third tier of the league system.
In the 2008–09 season, the club finished third, behind OFK Krušik and BSK Borča. They remained in the third tier until 2013–14, when they were promoted to the Serbian League West. During their second season in the second tier, the team was renamed FK Laktaši due to sponsorship reasons. They played in the lowest level of the league system for the next three seasons, before returning to the third tier in 2017–18.
Laktaši finished runners-up in the 2017–18 season, earning promotion to the Serbian First League. The club also competed in the 2018–19 Serbian First League of the East in the second tier of the league system, but finished the season bottom of the table and were subsequently relegated to the Serbian League West.
References
External links
Club page at Srbijasport
Club page at Srb
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