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CD3+ CD56+ cells that are also called natural killer T (NKT) cells are characterized by CD3+, CD16+ and CD56+ surface phenotype. It has been reported that CD3+CD56+ cells could be activated and expanded by cytokines, IL-2 and IL-15, and have the ability of cytotoxicity against NK cells. In this study, the activation and expansion of CD3+CD56+ cells were investigated in vitro and in vivo, following the recognition of alloantigens, by using a flow cytometry. The results showed that CD3+CD56+ cells were activated and expanded in a time-dependent manner, and those cells could become cytotoxic against NK cells. The inversional status of CD3+CD56+ cells was investigated, based on the activation and expansion of CD3+CD56+ cells by IL-2 and IL-15 in vitro, using inversional and non-inversional status as criteria. The results showed that inversional cells, even at a very early time, were detected in the peripheral blood and bone marrow of allograft recipients. By using antibodies against the NK and T cell surface receptors, inversional CD3+CD56+ cells were detected mainly on T lymphocytes. These data suggest that inversional status of CD3+CD56+ cells are caused by the recognition of alloantigens.
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* file that was distributed with this source code.
* An extra context that is specific to routing.
* @author Jean-François Simon
class RoutingContext implements RoutingContextInterface
public function __construct(LoggerInterface $