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I used to run spreadsheets on a regular basis, but I now use the same spreadsheet with 70% data columns and 30% calculations. So now when I want to change a small bit of data, I have to quit the formula editor and save the spreadsheet. Then when I’m done with the spreadsheet, I re-open it, find the formula I want and copy the value I want to the data column.
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The loss of the IIS component and its association with the MAPK pathway activation has been previously reported in thyroid tumors \[[@pone.0149245.ref039],[@pone.0149245.ref040]\]. However, these studies are conducted in cell-line models, in which the role of pERK protein was shown in a more simple model system, without the interference of other factors in the *in vivo* environment. For this reason, in our study, we were unable to quantify the MAPK pathway activation, but we were able to detect the increased level of the IIS components, which helps to unravel the alteration of cellular homeostasis that is observed in these tumors.
Our study confirms the occurrence of a distinct phenotype in pediatric brain tumors. In our work we were able to identify not only the expected expression patterns of some markers of the IIS, but also overexpression of TNSALP in the nuclear compartment, which is in line with a recent work by Bhand
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Please also see the Welcome Guide.Q:
Choosing an overhanging roof / underside support so that horizontal force is transferred to the roof loadbearing walls
I am designing a roof with a 10m overhanging roof overhang, and I’m not sure I have chosen the right supports to carry the roof load from below (see image).
Will this support arrangement transfer forces from the underside to the roof loadbearing walls, and if not why not?
The number you have is for a roof load of 36,000 kgs and a load of 6,000 kgs overhanging and assuming a 10m overhang, with an effective centre bearing of 27,000 kgs. This gives a 1 in 3 chance of catastrophic failure of the loadbearing walls.
Another thing to consider is the type of material you are using. Timber/metal roofs aren’t good in this case, they take much more than 1 in 3 failures. They will likely need to carry the entire load (excluding snow loads) and they will be carrying that load continuously. The material will be in compression, which will fatigue from time to time. If the roof material is damaged,