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To examine the role of protein kinase C-induced c-fos expression in the longevity of smooth muscle cells (SMCs), an inhibitor against c-fos expression was added to the culture medium of adult rat aorta SMCs, in which the duration of phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2) expression increases according to the increase in the duration of phorbol myristate acetate (PMA)-induced protein kinase C (PKC) activation. The c-fos-specific inhibitor was added to the culture medium of the PMA-stimulated SMCs. The duration of pERK1/2 expression and the % of pERK1/2-positive cells after an increase in the duration of PMA-induced PKC activation were measured. The PMA-induced pERK1/2 expression and PMA-induced % of pERK1/2-positive cells increased in proportion to the duration of PMA-induced PKC activation in the SMCs. The PMA-induced % of c-fos-positive cells increased after the induction of c-fos expression, which was strongly inhibited by treatment of the cells with the c-fos-specific inhibitor. The % of pERK1/2-positive cells decreased after the inhibition of c-fos expression, but that of pERK1/2-positive cells still increased in proportion to the duration of PMA-induced PKC activation. The c-fos-specific inhibitor suppressed
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